Structure of the glycine-rich, arginine-rich histone of the Novikoff hepatoma.
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چکیده
منابع مشابه
Incorporation of glycine into protien and nucleic acid fractions of nuclei of liver and hepatoma.
Proteins and nucleic acids of nuclei of liver and hepatoma in the rat \vere separated into three fractions: Fraction I, soluble in 0.05 Msodium citrate; Fraction II, extracted with 1 M NaCl; and Fraction III, the residual fraction. Histones, acid-insoluble pro teins, RNA, and DNA were found in each fraction. Specific activities of the protein and nucleic acid fractions of nuclei of liver and a ...
متن کاملA large scale procedure for isolation of the glycine-rich, arginine-rich histone and the arginine-rich, lysine-rich histone in a highly purified form.
1. A procedure is presented for large scale fractionation of arginine-rich histones by exclusion chromatography. With this method, 2 to 3.5 g of histone Fractions 2a and 3 were fractionated. The two fractions contained the same histone components, but they differed in the amounts of each component. 2. In histone Fraction 2a, the glycine-rich, arginine-rich (GAR) histone accounted for approximat...
متن کاملThe fractionation of arginine-rich histones from fetal calf thymus by exclusion chromatography.
The arginine-rich histone complex from fetal calf thymus was found to contain 12 or more histone components by electrophoretic analysis on polyacrylamide gels. The arginine-rich histone complex was resolved into five distinct peaks b,y exclusion chromatography on Sephadex G-100 columns 300 cm in height and 7.6 cm in diameter, with 0.01 M HCl as the eluent. Of the five peaks obtained, two (Peaks...
متن کاملStructural analysis of the glycine-rich, arginine-rich histone. II. Sequence of the half of the molecule containing the aromatic amino acids.
1. The glycine-rich, arginine-rich histone from calf thymus was hydrolyzed with chymotrypsin; the peptides produced were fractionated on Sephadex G-25. The peptides containing the aromatic amino acids were purified by rechromatography on Sephadex G-25 and Aminex A-4 columns and by peptide mapping. 2. The six aromatic amino acids of the glycine-rich, arginine-rich histone were accounted for in e...
متن کاملProtein and cDNA sequence of a glycine-rich, dimethylarginine-containing region located near the carboxyl-terminal end of nucleolin (C23 and 100 kDa).
By a combination of protein chemistry and recombinant DNA methods a glycine-rich region was found to be located near the carboxyl terminus of the nucleolar specific phosphoprotein, nucleolin, from Novikoff hepatoma (protein C23) and Chinese hamster ovary cells (100-kDa nucleolar protein). A sequence of 192 amino acid residues was derived from partial sequences of cyanogen bromide and N-bromosuc...
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ورودعنوان ژورنال:
- Cancer research
دوره 30 12 شماره
صفحات -
تاریخ انتشار 1970